Studies on the pathogenesis of hemorrhagic fever with renal syndrome(HFRS) have been seriously hindered by the absence of appropriate animal model. the major pathologic findings of HFRS are generalized vasculopathy, increased permeability of capillaries, abnormal functions of various organs, and disseminated intravascular coagulation. The necrosis of tubules followed by the vasculopathy of the kidney, especially capillaries has been-suggested as the pathogenesis of acute renal failure (ARF) in HERS. The presence of specific antibodies against Hantaan virus was demonstrated in the endothelium of glomerular capillaries- and basement membranes of tubules in the kidney of HFRS patients. Virus particles were also detected in the renal tubular epithelial cells. Suckling mice were shown to be infected by Hantaann virus resulting in the production of neurological symptoms. This suckling mice model has been applied for the infectivity assay. However, ARF and viral particles have not been observed in tubular epithelial cells from infected suckling mice. De-position of non-specific immune complex and increased blood concentrations of urea nitrogen´ and creatinine indicated abnormal functins of kidney. The objectives of this study are to determine the growth of Hantaan virus in ce14 cultures of the renal tubular epithelial cells from infected suckling
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mice, and to compare the pathogenesis of abnormal renal functions in the animal model with that of
{ human patients. In this study, cultured murine renal tubular cells were used to simplify the assay for -viral infectivity and to overcome the complexties of in vivo assay system. Renal tubular cells were obtained from one day-old suckling mice, and these cells were differentiated from one day
old suckling mice, and these cells were differentiated from vascular endothelial cells by the absence of coagulation factor VIII. As the results, the renal cells cultured in this experiment were almost tubular cells. The cultured renal tubular cells were infected with Hantaan virus (Strain 76-118). The outcome of Hantaan virus infection of cultured renal cells was assessed by fluorescence activated; cell scanning(FAGS). This FACS assay indicated that 43% of the cells contained viral antigen within 24 hours postinfection. the proportion of virus-containing cells increased to 80% on the day of the third, 83% on the fifth, 86% on the seventh, and 83% on the ninth, respectively. The renal tubular cells infected with Hantaan virus 76-118 were not shown cytopathic effects. But the bind-
ing activity of merocyanine 540 in the renal tubular cells infected with Hantaan virus were higher than control.
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